Smikodoub Alexander, Novytskaja Alla
Cell Therapy Clinic of National Medical University and Embryonic Tissue Center "EmCell"

The transplantations of fetal stem cells were made to 11 diabetes mellitus patients, complicated with diabetic glomerulosclerosis, chronic renal insufficiency, for the erythropoiesis reconstitution.

The indication for the transplantation was anemia of different range, resistant to conventional hemostimulating therapy (iron preparations, ziankobalomine, Folic acid). The group consisted of patients of 25 to 46 years old (average 33.5 years old). 6 patients had chronic renal insufficiency of the I range (creatinine - 0.175+0.019 mmol/1, urea - 10.13+LO mmol/1, bulb filtration reaction speed - 56.17+3.69 ml/min; erythrocytes of peripheral blood - 2.94+0.12xl012/!, hemoglobin - 92.5+5.4 g/1). 5 patients had chronic renal insufficiency of II range (creatinine - 0.326+0.066 mmol/1, urea - 15.97+0.68 mmol/1, bulb filtration reaction speed - 34.2+3.34 ml/min; erythrocytes of peripheral blood - 2.26+0.10xl012/!, hemoglobin - 75.6+1.91 g/1).

There were administered (intravenous dropwise once) cells suspensions with the following parameters: age - 5-12 weeks of gestation; suspension volume - from 1 to 4 ml; CD34 number - from 0.3 to 2.9xl06/ml; number of CFU-GEMM - from 2.6 to 23xl03/ml.

We observed:

- gradual rising of hemoglobin and erythrocytes level, starting from 5-7 day, with the maximum by 1-1.5 month (erythrocytes - 3.84+0.12xl012/! and 3.38+0.21xl012/!; hemoglobin - 118.8+4.76 g/1 and 103.6+4.4 g/1 respectively in the I and II groups). The effect continuance in the I group - from 5 to 11 months, in the II - from 2 to 5 months.

- functional kidney state improvement: daily proteinuria reduction, urea sediment improvement, moderate rising of bulb filtration speed.

- arterial pressure level reduction during 4-6 months with the simultaneous re- duction of hypotensive medication dose.

- recipients' immune reactivity parameters reconstitution, that was clinically manifested in the reduction of frequency and expressiveness of viral infections, complications of chronic inflammatory diseases (chronic pyelonephritis, chronic bronchitis, etc.).

Smikodoub Alexander, Novytskaja Alla Treatment of patients with diabetes mellitus complicated with diabetic glomerulosclerosis, chronic renal insufficiency by fetal stem cells. Society for Minimally Invasive Therapy Ninth Annual International Meting 14-16 July 1997, Kyoto, Japan. Minimally Invasive Therapy. 1997, v.5, s.1, p.39.