Novytskaja Alla, Smikodoub Alexander
Cell Therapy Clinic of National Medical University and Embryonic Tissues Center "EmCell"

20 patients aged from 1 to 35, with clinical diagnosis of initially revealed insulin- dependent sugar diabetes, were subjected to transplantation of human fetal cells possessing hypoglycemic and immunocorrecting effect.

Suspension characteristics were as follows:

- embryo age was 5 to 9 weeks of gestation;
- suspension composition: stem cells of human fetal hemopoiesis and hepatocytes;
- suspension amount: 1 to 4 ml;
- amount of CD34: 0.28 to 0.8*106/1111;
- amount of CPU GEMM: 2.1 to 9* 103/ml.

Method of administration: intravenous, drop-by-drop. Number of administra- tions: 14 patients were' subjected to 1 transplantation each, and 6 patients were subjected to 2 transplantations each, intervals between transplantations being 2 to 10 months.

Transplantations were carries out 1 to 6 months after making the diagnosis. Duration of observation was 1 to 4 years. Results:

l. In all patients, 30 to 80 % decrease in the daily dosage of administered insulin was observed (45 % for the total group); the peak of such decrease was noted after 14 to 90 days.

2. Clinical remission (daily dosage of insulin of 0.4 units/kg of body mass or complete cancellation of insulin administration) developed in 65 % of patients. Possible is subsequent administration of cell suspension to prolong the term of remission (repeated administration to 6 patients).

3. Restoration of normal immune status indices was observed on the 11th to 30th day.

No side effects or allergic reactions were noted.

Efficiency of cell therapy in case of initially revealed insulin-dependent sugar diabetes depends, on the disease duration: the earlier transplantation is carried out, the more probable is development of clinical remission.

Novytskaja Alla, Smikodoub Alexander Treatment of patients with initially revealed insulin dependent sugar diabetes with the USE of fetal cell suspensions. Society for Minimally Invasive Therapy Eighth International Meeting. 18-20 September 1996, Villa Erba, Cernobbio, Italy. Minimally Invasive Therapy. 1996, v.5, s.1, p.153.