Smikodub A.I.
Cell Therapy Clinic of National Medical University, Embryonic Tissues Center EmCell

Analyzed is the experience of embryonic cell suspension treatment (ECS) of 107 cancer patients with the following diseases: hemoblastosis, stomach cancer, intestine cancer, cervical and uterine cancer, osteogenic sarcoma and Wilms’ tumor. Embryonic stem cells were obtained from human embryonic liver and spleen of the 6-12th week of gestation. The quantity of nucleated cells varied from 30 till 100x10*6. Suspensions were administered intravenously, dropwise in the volume from 1 to 5 ml. Its functional activity was evaluated in accordance with the quantity of CFU-GM, CFU-GEMM, and CD 34. ECS were applied at 3 stages of treatment of cancer patients: 1) prior to special anti-tumor treatment (surgery, chemotherapy, or radiation) aimed at providing the possibility of the above mentioned methods by avoiding of possible complications (anemia, leukopenia, cachexia, etc.); 2) during the course of chemotherapy or radiation with the aim of treatment of cytostatic myelodepression syndrome; 3) in the course of remission with the aim to secure the achieved results. After the transplantation of ECS to patients with different complications of malignant formations hindering the beginning of specialized treatment, there was noted restoration of hemapoiesis, increase of Karnofsky’s Index, decrease of the development of clinical complications, which gave the patient the possibility to begin with surgical treatment or chemotherapy in 2-4 weeks. In patients with cytostatic myelodepression syndrome, restoration of the quantity of leukocytes was noted on the 3-7th day, erythrocytes – on the 10-14th day, thrombocytes – on the 10-20th day. Increase of the total quantity of lymphocytes, T-lymphocytes, CD4 subpopulations, NK-cells by 20-100% was noted after administration of ECS both within the period of cytostatic treatment and in the course of remission. In 70% of cases there was noted improvement of long-term results of the treatment.

Smikodub A.I. Embryonic Stem Cell Transplantation in Anticancer Treatment. Abstracts of the 13th International Symposium, Copenhagen, Denmark. June 14-16 2001. Supportive Care in Cancer - Official Journal of Multinational Association of Supportive Care in Cancer - Vol.9 No.4 June 2001, P - 128