A. Smikodub, N. Snegir.
Cell Therapy Clinic of National Medical University Embryonic Tissues Center "EmCell"

Neutropenia is an important fact that gives an impulse to infectious complications of oncological patients upon chemotherapy. In this work fetal stem cells were used for treatment of great neutropenia (less than 500 in 1 ml).Cell suspensions were collected from a liver of one embryo of the gestation term from 6 to 12 weeks. Cryopreservation was carried out according to the special program with DMSO. Prenatal and fetal infection diagnostics, bacteriological control were provided. CD-34, CFU-GM and CFU-GEMM were rated. Fetal stem cells were injected intravenous drop-wise once, or several times Suspensions volume was from 1 to 4 ml, cells number 30-250x106/1 ml.

11 patients with acute leucosis were under observation. Neutropenia has expanded for less than 500 in 1 ml at all of them during the treatment. At the time of transplantation febrile neutropenia persisted among 6 patients. Among 4 patients its durability against the antibacterial therapy was 7 days, among 2 patients - more than 7 days. Among 3 patients the focus of infection was not determined, one patient had a clinical aspect of sepsis, another one had oral mucositis and pneumonia. After fetal stem cell suspensions injection, among 4 patients, there was a syndrome of earlyposttranspalntation improvement, manifested in weakness reduction, lustiness, appetite rise (Smikodub's syndrome).

Among 4 patients, with febrility up to 2-7 days after fetal stem cells injection, temperature reduction was noticed on the 1st day and its normalization - on 2-3rd day. The patient with sepsis was noticed to have fever reduction up to subfebrile figures, with periodic febrile rises. One patient's temperature had normalized on the 11th day after cell suspension injection.

The positive dynamics of patients' state corresponded with positive dynamics of peripherial blood parameters: leukocytes number had increased on 3-5th day and restored on 8-10th day.

Smikodub A., N. Snigir. FEBRILE NEUTROPENIA TREATMENT OF PATIENTS WITH ACUTE LEUCOSIS BY FETAL STEM CELLS. Abstracts of the 3rd International Symposium on Febrile Neutropenia. Brussels, Belgium, December 10-13, 1997. Supportive Care in Cancer, 1998, vol. 6, N 3, p. 326.